ABSTRACT
Objective:To investigate the relationships between the levels of serum interleukin-17 (IL-17),interleukin-33 (IL-33) and the concentration of fractional exhaled nitric oxide (FeNO) in the patients with chronic cough,and to further evaluate their effects in the pathogenesis of chronic cough.Methods:A total of 160 patients diagnosed with chronic cough for more than 8 weeks were chosen and used as chronic cough group.At the same time,60 healthy controls received physical examination were selected as healthy control group.The levels of serum IL-17 and IL-33 of the subjects were examined and pulmonary function test,FeNO concentration test and comprehensive allergen test were performed.All data were analyzed by GraphPad Prism statistical software.The levels of serum IL-17 and IL-33 were compared between chronic cough group and healthy control groups.The correlations between the levels of serum IL-17 or IL-33 and lung function or FeNO in the patients with chronic cough were further analyzed.Results:The serum IL-17 and IL-33 levels of the patients in chronic cough groups were higher than those in healthy control group (P＜0.05).The serum IL-17 and IL-33 levels in the patients with chronic cough showed a significant negative correlations with the percentage of the first second force expiratory volume to the estimated value (FEV1％) of the patients (r=-0.624 5,r=-0.672 2),and the level of serum IL-33 in the patients with chronic cough was positively correlated with the concentration of FeNO;the higher the concentration of FeNO,the higher the level of serum IL-33 (rs =0.758,P＜0.05).The FeNO concentration geometric mean of the patients with the serum total IgE＜100 IU · mL-1 (33 ppb) was significantly lower than those with the serum total IgE＞200 IU · mL-1 (78 ppb) and IgE 100-200 IU · mL 1 (69 ppb) among the chronic cough patients (P＜0.01),but there was no significant difference between the later two groups (P=0.082 4).Conclusion:The serum IL-17 and IL-33 may play an important role in the pathogenesis of chronic cough as proinflammatory factors.The levels of serum IL-17 or IL-33 have negative correlations with the pulmonary ventilation function in the patients with chronic cough.The increasing of serum IL-33 level may predict the formation of eosinophilic airway inflammation and reflect the severity of eosinophilic airway inflammation.
ABSTRACT
Objective:To establish an HPLC method for the determination of genistein in genistein-loaded Eudragit nanoparticles. Methods:The HPLC analysis was carried out on a DiamonsilTM C18(250 mm ×4.6 mm,5μm)column with the mobile phase of meth-anol-water (85∶15) at the flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 261nm and the column temperature was 25℃. Results: A good separation was displayed between the drug peak and the excipient peaks. An excellent linearity range for genistein was obtained from 1.0 μg·ml-1 to 100.0 μg·ml-1(r =0.999 8). The recovery of genistein was 99.85%(RSD =0. 65%,n=9). Conclusion:The method is simple, rapid, accurate and specific, which is suitable for the determination of content and encapsulation efficiency of genistein-loaded nanoparticles.